簡要描述：LONZA PYROGENT-5000 100 Test Kit
PYROGENT -5000產(chǎn)品適合用于實驗室處理大量樣品，可以定量的動態(tài)檢測革蘭氏陰性細菌內(nèi)毒素。檢測在96孔板上進行，允許許多樣品同時檢測。The PYROGENT™–5000 assay是水樣，大量注射用藥物和醫(yī)療設備沖洗水內(nèi)毒素檢測的理想選擇。檢測使用溫育板讀數(shù)器340nm讀數(shù)，檢測靈敏度在0.01 to 100.0 EU

產(chǎn)品英文名稱：PYROGENT™–5000 Kinetic Turbidimetric LAL Assays
產(chǎn)品中文名稱：PYROGENT™–5000動態(tài)濁度法內(nèi)毒素檢測試劑盒

PYROGENT™–5000 Kinetic Turbidimetric LAL Assays產(chǎn)品介紹：
PYROGENT -5000產(chǎn)品適合用于實驗室處理大量樣品，可以定量的動態(tài)檢測革蘭氏陰性細菌內(nèi)毒素。檢測在96孔板上進行，允許許多樣品同時檢測。The PYROGENT™–5000 assay是水樣，大量注射用藥物和醫(yī)療設備沖洗水內(nèi)毒素檢測的理想選擇。檢測使用溫育板讀數(shù)器340nm讀數(shù)，檢測靈敏度在0.01 to 100.0 EU/ml。

檢測原理：樣品和LAL（鱟變形細胞裂物）反應物混合，革蘭氏陰性細菌內(nèi)毒素催化LAL酶原反應，反應初速度決 定于內(nèi)毒素濃度。激活的凝固酶原水解LAL凝固蛋白酶原特定化學鍵形成凝固蛋白。水解形成的凝固蛋白會立即自我凝集成膠狀物。LAL濁度法檢測可以測定濁 度增加值（光密度），濁度變化在凝膠形成之前出現(xiàn)。反應物變混濁所需時間（反應時間）與細菌內(nèi)毒素含量成反比例。也就是說，如果有大量內(nèi)毒素存在，則反應 迅速，很快就會出現(xiàn)混濁；如果有少量內(nèi)毒素存在反應時間就會延長。未知樣品內(nèi)毒素濃度可通過標準曲線計算出。

注意事項：
¡ 本品為體外診斷試劑，嚴禁用于人體內(nèi)毒素檢測
¡ 本品用于藥品的G-細菌內(nèi)毒素檢測時，應遵循USP藥典細菌內(nèi)毒素檢測法的規(guī)定
¡ 如果說明書下載不了或者有其它產(chǎn)品信息問題可以咨詢

PYROGENT™–5000 Kinetic Turbidimetric LAL Assays英文介紹：
The PYROGENT™–5000 assay is perfect for laboratories needing to process large numbers of samples. This assay is performed in a 96-well plate allowing many samples to be processed at one time. It is ideal for water samples, large volume parenterals and water rinse from medical devices. The PYROGENT™–5000 assay is measured at the 340 nm wavelength and has a sensitivity range from 0.01 to 100.0 EU/ml. An incubating plate reader is required to run this assay.

PYROGENT -5000 is a quantitative, kinetic assay for the detection of Gram negative bacterial endotoxin. A sample is mixed with the reconstituted LAL reagent, placed in the photometer, and automatically monitored over time for the appearance of turbidity. The time required before the appearance of turbidity (Reaction Time) is inversely proportional to the amount of endotoxin present. That is, in the presence of a large amount of endotoxin the reaction occurs rapidly; in the presence of a smaller amount of endotoxin the reaction time is increased. The concentration of endotoxin in unknown samples can be calculated from a standard curve.

 訂購信息：
N383(Cat.No.)  2瓶/盒(鱟試劑)   100Tests   5.2ml/瓶(需混合的LAL重配緩沖劑體積)
N384(Cat.No.)  2瓶/盒(鱟試劑)   200Tests   10.4ml/瓶(LAL重配緩沖劑體積)
N588(Cat.No.)  45瓶/盒(鱟試劑)  2250Tests  5.2ml/瓶(LAL重配緩沖劑體積)
N688(Cat.No.)  45瓶/盒(鱟試劑)  4500Tests  10.4ml/瓶(LAL重配緩沖劑體積)