簡(jiǎn)要描述：RHB-Basa 神經(jīng)干細(xì)胞培養(yǎng)基,NS定向分化
Cellartis RHB-A和RHB-Basal是兩款無(wú)血清、成分*確定的培養(yǎng)基，用于人類或小鼠神經(jīng)干細(xì)胞（NS）的定向分化獲取、維持培養(yǎng)、擴(kuò)增、誘導(dǎo)分化成神經(jīng)細(xì)胞。

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英文名稱：Cellartis RHB-Basa Stem Cell Culture Media，Cellartis RHB-A Stem Cell Culture Media

中文名稱：Cellartis RHB-Basa神經(jīng)干細(xì)胞培養(yǎng)基，Cellartis RHB-A神經(jīng)干細(xì)胞培養(yǎng)基  

RHB-A和RHB-Basal是兩款無(wú)血清、成分*確定的培養(yǎng)基，用于人類或小鼠神經(jīng)干細(xì)胞（NS）的定向分化獲取、維持培養(yǎng)、擴(kuò)增、誘導(dǎo)分化成神經(jīng)細(xì)胞等用途。RHB-A可用來(lái)培養(yǎng)擴(kuò)增貼壁的人或小鼠的神經(jīng)干細(xì)胞。在RHB-A中培養(yǎng)的神經(jīng)干細(xì)胞保持著穩(wěn)定的神經(jīng)干細(xì)胞分化發(fā)育成為成熟的神經(jīng)細(xì)胞的能力 (1-3) 。另外，RHB-A還用來(lái)定向分化小鼠胚胎干細(xì)胞(mouse ES)至神經(jīng)前體細(xì)胞 (neural precursors) (4-6)。

RHB‐Basal不包含生長(zhǎng)因子或是神經(jīng)細(xì)胞相關(guān)的添加物。因此，可以RHB‐Basal為基礎(chǔ)，通過(guò)添加客戶認(rèn)為需要的添加物來(lái)開(kāi)發(fā)適合客戶的特殊類型細(xì)胞的培養(yǎng)基。

RHB-A and RHB-Basal is a fully defined, serum-free cell culture medium for the derivation, maintenance, expansion, and differentiation of human and mouse neural stem (NS) cells. RHB-A is specifically formulated for culturing pure populations of adherent human and mouse NS cells. This optimized medium ensures the long-term stability of NS cell differentiation capacity (1) and can be used to support differentiation of human and mouse NS cells into functional neurons (1–3) and of mouse ES cells into neural precursors (4–6).

RHB‐Basal does not contain any growth factors or neuronal supplements.  Therefore, this media can be tailored to the specific requirements of your cell type by the addition of supplements.

產(chǎn)品特點(diǎn)：(NS細(xì)胞：神經(jīng)干細(xì)胞）

RHB-A是一種專有的成分確定的無(wú)血清培養(yǎng)基，用于維持人類和小鼠貼壁NS細(xì)胞群培養(yǎng)

RHB‐Basal不含動(dòng)物成分，不含神經(jīng)元添加劑；培養(yǎng)基可以通過(guò)補(bǔ)充添加劑來(lái)定制

RHB-A is a proprietary, fully defined, and serum-free medium designed to maintain pure populations of adherent human and mouse NS cells

RHB-Basal medium is animal component-free and contains no neuronal supplements; the media can be customized by the addition of supplements

產(chǎn)品應(yīng)用(ES：胚胎干細(xì)胞）

從ES細(xì)胞和胎兒和成人組織中分離培養(yǎng)小鼠和人類NS細(xì)胞

小鼠和人類貼壁NS細(xì)胞的維持和增殖

小鼠和人類NS細(xì)胞分化為功能性神經(jīng)元

小鼠ES細(xì)胞向神經(jīng)元前體的分化

有關(guān)使用的其他示例，請(qǐng)參閱數(shù)據(jù)表

Derivation of mouse and human NS cells from ES cells and fetal and adult tissues

Maintenance and propagation of adherent mouse and human NS cells

Differentiation of mouse and human NS cells into functional neurons

Differentiation of mouse ES cells to neuronal precursors

Refer to the Data Sheet for additional examples of use

組份：

500 ml medium

參考文獻(xiàn)：

1. Sun Y, Pollard S, Conti L, Toselli M, Biella G, Parkin G, Willatt L, Falk A, Cattaneo E, and Smith A. (2008) Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture. Molecular and Cellular Neuroscience. 38: 245-258.

2.Conti L, Pollard SM, Gorba T, Reitano E, Toselli M, Biella G, Sun Y, Sanzone S, Ying QL, Cattaneo E, and Smith A. (2005) Niche-Independent symmetrical self-renewal of a mammalian tissue stem cell. PLoS Biology. 3(9): e283.

3.Pollard SM, Conti L, Sun Y, Goffredo D, and Smith A. (2006) Adherent Neural Stem (NS) cells from fetal and adult forebrain. Cerebral Cortex. 16: 112-120.

4.Ying QL, Stavridis M, Griffiths D, Li M, and Smith A. (2003) Conversion of embryonic stem cells into neuroectodermal precursors in adherent mono-culture. Nature Biotechnology. 21: 183-186.

5.Pollard SM, Wallbank R, Tomlinson S, Grotewold L, and Smith A. (2008) Fibro-blast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors and during embryonic stem cell differentiation. Molecular and Cellular Neuroscience. 38: 393-403.

6.Pollard SM, Yoshikawi K, Clarke ID, Danovi D, Stricker S, Russell R, Bayani J, Head R, Lee M, Bernstein M, Squire J, Smith A, and Dirks P. (2009) Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens. Cell Stem Cell. 4: 568-580.

7.Diogo MM, Henrique D, and Cabral JM. (2008) Optimization and integration of expansion and neural commitment of mouse embryonic stem cells. Bio-technology and Applied Biochemistry. 49: 105-112.

8.Abranches E, Silva M, Pradier L, Schulz H, Hummel O, Henrique D, and Bek-man E. (2009) Neural differentiation of embryonic stem cells in vitro: A road map to neurogenesis in the embryo. PLoS ONE. 4(7): e6286.

9.Hansen DV, Lui JH, Parker PRL, and Kriegstein AR. (2010) Neurogenic radial glia in the outer subventricular zone of human neocortex. Nature. 464(7288): 554-561.

華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司主營(yíng)細(xì)胞治療和精準(zhǔn)醫(yī)療等產(chǎn)品，2018年與TAKARA繼續(xù)合作，授權(quán)代理（上海）其旗下Cellartis干細(xì)胞研究制品和Rubicon單細(xì)胞文庫(kù)構(gòu)建試劑。Cellartis公司位于瑞典，擁有iPS細(xì)胞等干細(xì)胞分化為肝細(xì)胞及臟器細(xì)胞的分化誘導(dǎo)技術(shù)和ES細(xì)胞，ips細(xì)胞，分化細(xì)胞等干細(xì)胞相關(guān)產(chǎn)品。

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